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PHYS 543 - Biomedical Optics

Course Outline:

I. Introduction

1.1. Light-tissue interaction - initial events, diagnostic applications

Absorption

Elastic Scattering

Inelastic (Raman) Scattering

Fluorescence

1.2. Light-tissue interaction - effects on tissue, therapeutic applications

Photothermal

Photomechanical

Photochemical

II. Radiative Transport Theory

2.1. Optical transport parameters - m_a, m_s, g

2.2. Propagation of light in tissue

Radiometry

Transport equation

Diffusion Theory

2.3. Optical properties of tissue

Methodology of measurements

Tissue chromophores - melanin, hemoglobin, bilirubin

Transport parameters of biological tissues

2.4. Monte Carlo Simulation

Light distribution in tissue/Reflectance measurements

Fluorescence measurements/Raman measurements

III. Tissue Spectroscopy

3.1. Instrumentation

Optical fibers

Light sources

Spectrograph/monochromators

Detectors (1D & 2D)

3.2. Reflectance spectroscopy

3.3. Fluorescence spectroscopy

3.4. Raman spectroscopy

IV. Optical Imaging of Tissue

4.1. Reflectance and polarizing imaging

4.2. Ultrasound modulated optical imaging

4.3. Photoacoustic imaging

4.3. Fluorescence imaging

4.4. Time resolved imaging (time domain & frequency domain)

4.5. In vivo confocal imaging

4.6. Multiphoton excitation imaging (fluorescence/SHG)

4.7. Optical coherence tomography (OCT)

4.8. Lab demonstration of tissue imaging and spectroscopy

V. Therapeutic and Theranostics Applications

5.1. Endoscopy

5.2. Photodynamic therapy (PDT)

5.3. UV based phototherapy

5.4. Selective photothermolysis - Laser Therapy in Dermatology

5.5. Multiphoton absorption based photothermolysis